Recombinant Human Anti-TSHR Antibody (M22) (CAT#: PABW-172)
Autoantibody
Recombinant Human Antibody (M22) is capable of binding to TSHR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-TSHR mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- TSHR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. A human monoclonal autoantibody to the TSHR (M22), which is a powerful thyroid stimulator with high affinity for the receptor, and determined its crystal structure at 1.65A° resolution.
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-TSHR Antibody (PABW-172) in SDS-PAGE
SDS-PAGE analysis of PABW-172 in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Recombinant Human Anti-TSHR Antibody (PABW-172) in HPLC
HPLC analysis of PABW-172 shows the purity is>95%.
DB
Figure 3 Recombinant Human Anti-TSHR Antibody (PABW-172) in Dot Blot
Dot Blot analysis of PABW-172 was performed by coating with human TSHR antigen protein.
PABW-172 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 4 Recombinant Human Anti-TSHR Antibody (PABW-172) in ELISA
ELISA analysis of PABW-172 was performed by coating with recombinant human TSHR antigen. Then blocked with BSA, and incubated with Recombinant Human Anti-TSHR Antibody (M22) (PABW-172). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Human thyroid stimulating hormone receptor
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native TSHR
- Species Reactivity
- Human
- Clone
- M22
- Applications
- WB, ELISA, PK
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The TSHR antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Pharmacokinetic.
Target
- Alternative Names
- TSHR; thyroid stimulating hormone receptor; LGR3; CHNG1; hTSHR-I; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2
- Gene ID
- 7253
- UniProt ID
- P16473
Related Resources
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-353 | Mouse Anti-TSHR Recombinant Antibody scFv Fragment (PSBL-353) | WB, ELISA, FuncS | Mouse scFv |
| PSBW-172 | Human Anti-TSHR Recombinant Antibody (clone M22); scFv Fragment | WB | Human scFv |
| FAMAB-0323CQ-S(P) | Mouse Anti-TSHR Recombinant Antibody (clone 4G11); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0328CQ-S(P) | Mouse Anti-TSHR Recombinant Antibody; scFv Fragment (HPAB-0328CQ-S(P)) | ELISA, FC, FuncS | Mouse scFv |
| HPAB-0329CQ-S(P) | Mouse Anti-TSHR Recombinant Antibody; scFv Fragment (HPAB-0329CQ-S(P)) | ELISA, FC, FuncS, Agonist | Mouse scFv |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABW-172, RRID: AB_3111691)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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