Recombinant Human Anti-TSHR Antibody (M22)

CAT#: PABW-172 Autoantibody

Recombinant Human Antibody (M22) is capable of binding to TSHR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-TSHR mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- TSHR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. A human monoclonal autoantibody to the TSHR (M22), which is a powerful thyroid stimulator with high affinity for the receptor, and determined its crystal structure at 1.65A° resolution.

Tested Data Gene Expression
Figure 1 Recombinant Human Anti-TSHR Antibody (PABW-172) in SDS-PAGE Figure 2 Recombinant Human Anti-TSHR Antibody (PABW-172) in HPLC Figure 3 Recombinant Human Anti-TSHR Antibody (PABW-172) in Dot Blot Figure 4 Recombinant Human Anti-TSHR Antibody (PABW-172) in ELISA
Figure 1 Thyroid gland Figure 2 Testis Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, SCLC-21H, U-266/70) Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, SCLC-21H, U-266/70)

Specifications

  • Immunogen
  • Human thyroid stimulating hormone receptor
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native TSHR
  • Species Reactivity
  • Human
  • Clone
  • M22
  • Applications
  • WB, ELISA, PK

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The TSHR antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Pharmacokinetic.

Target

  • Alternative Names
  • TSHR; thyroid stimulating hormone receptor; LGR3; CHNG1; hTSHR-I; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2
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Citations

  1. Rotondo Dottore, Giovanna, et al. "Insights into the role of DNA methylation and gene expression in graves orbitopathy." The Journal of Clinical Endocrinology & Metabolism 108.5 (2023): e160-e168. https://doi.org/10.1210/clinem/dgac645
    This study investigates the role of DNA methylation and gene expression in Graves orbitopathy (GO). Researchers examined orbital fibroblasts from both GO patients and control subjects, focusing on DNA methylation under basal conditions and after exposure to an anti-thyrotropin (TSH) receptor antibody (M22) and various cytokines. The results showed a significant increase in global DNA methylation in both control and GO fibroblasts when incubated with M22. Additionally, the study identified numerous differentially methylated DNA regions and correlated these changes with altered gene expression and increased cell proliferation. The findings suggest that differential DNA methylation and gene expression in orbital fibroblasts contribute to the pathological features of GO.
    Creative Biolabs provided the human monoclonal anti-TSHR antibody M22 (Cat#: PABW-172) for this study. The M22 antibody was crucial in demonstrating the link between TSHR activation and increased DNA methylation, gene expression changes, and cell proliferation in orbital fibroblasts. This contribution was essential for elucidating the epigenetic mechanisms underlying the persistent functional alterations observed in GO, thereby advancing the understanding of the disease's pathogenesis.

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(Creative Biolabs Cat# PABW-172, RRID: AB_3111691)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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