Recombinant Human Anti-TSHR Antibody (M22)
CAT#: PABW-172 Autoantibody
Recombinant Human Antibody (M22) is capable of binding to TSHR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-TSHR mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- TSHR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. A human monoclonal autoantibody to the TSHR (M22), which is a powerful thyroid stimulator with high affinity for the receptor, and determined its crystal structure at 1.65A° resolution.








Specifications
- Immunogen
- Human thyroid stimulating hormone receptor
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native TSHR
- Species Reactivity
- Human
- Clone
- M22
- Applications
- WB, ELISA, PK
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The TSHR antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Pharmacokinetic.
Target
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Citations
-
Rotondo Dottore, Giovanna, et al. "Insights into the role of DNA methylation and gene expression in graves orbitopathy." The Journal of Clinical Endocrinology & Metabolism 108.5 (2023): e160-e168. https://doi.org/10.1210/clinem/dgac645This study investigates the role of DNA methylation and gene expression in Graves orbitopathy (GO). Researchers examined orbital fibroblasts from both GO patients and control subjects, focusing on DNA methylation under basal conditions and after exposure to an anti-thyrotropin (TSH) receptor antibody (M22) and various cytokines. The results showed a significant increase in global DNA methylation in both control and GO fibroblasts when incubated with M22. Additionally, the study identified numerous differentially methylated DNA regions and correlated these changes with altered gene expression and increased cell proliferation. The findings suggest that differential DNA methylation and gene expression in orbital fibroblasts contribute to the pathological features of GO.
Creative Biolabs provided the human monoclonal anti-TSHR antibody M22 (Cat#: PABW-172) for this study. The M22 antibody was crucial in demonstrating the link between TSHR activation and increased DNA methylation, gene expression changes, and cell proliferation in orbital fibroblasts. This contribution was essential for elucidating the epigenetic mechanisms underlying the persistent functional alterations observed in GO, thereby advancing the understanding of the disease's pathogenesis.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABW-172, RRID: AB_3111691)
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Related Diseases
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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TAB-192CQ | Mouse Anti-TSHR Recombinant Antibody (TAB-192CQ) | ELISA, WB, Inhib | Mouse IgG |
TAB-189CQ-S(P) | Mouse Anti-TSHR Recombinant Antibody; scFv Fragment (TAB-189CQ-S(P)) | ELISA, BL | Mouse scFv |
TAB-190CQ-S(P) | Mouse Anti-TSHR Recombinant Antibody; scFv Fragment (TAB-190CQ-S(P)) | ELISA, BL | Mouse scFv |
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TAB-191CQ-F(E) | Human Anti-TSHR Recombinant Antibody; Fab Fragment (TAB-191CQ-F(E)) | ELISA, WB, Inhib | Human Fab |
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